MersV1, Main, Exploration, bibRecord, 003E28

Differentiation of Acinetobacter baumannii biotypes by amplification of 16S-23S rRNA intergenic spacer sequences.

Identifieur interne : 003E28 ( Main/Exploration ); précédent : 003E27; suivant : 003E29

Differentiation of Acinetobacter baumannii biotypes by amplification of 16S-23S rRNA intergenic spacer sequences.

Auteurs : A. Garcia [Chili] ; R. Montoya ; H. Bello ; G. Gonzalez ; M. Dominguez ; R. Zemelman

Source :

Microbios [ 0026-2633 ] ; 1996.

RBID : pubmed:9141712

Descripteurs français

KwdFr :
- ADN bactérien (génétique), ADN ribosomique (génétique), Acinetobacter (), Acinetobacter (génétique), Génotype, Humains, Infection croisée (microbiologie), Infections à Acinetobacter (microbiologie), Infections à Acinetobacter (épidémiologie), Technique RAPD, Techniques de typage bactérien.
MESH :
- génétique : ADN bactérien, ADN ribosomique, Acinetobacter.
- microbiologie : Infection croisée, Infections à Acinetobacter.
- épidémiologie : Infections à Acinetobacter.
- Acinetobacter, Génotype, Humains, Technique RAPD, Techniques de typage bactérien.

English descriptors

KwdEn :
- Acinetobacter (classification), Acinetobacter (genetics), Acinetobacter Infections (epidemiology), Acinetobacter Infections (microbiology), Bacterial Typing Techniques, Cross Infection (microbiology), DNA, Bacterial (genetics), DNA, Ribosomal (genetics), Genotype, Humans, Random Amplified Polymorphic DNA Technique.
MESH :
- chemical , genetics : DNA, Bacterial, DNA, Ribosomal.
- classification : Acinetobacter.
- epidemiology : Acinetobacter Infections.
- genetics : Acinetobacter.
- microbiology : Acinetobacter Infections, Cross Infection.
- Bacterial Typing Techniques, Genotype, Humans, Random Amplified Polymorphic DNA Technique.

Abstract

Isolates of Acinetobacter baumannii (32 strains) from blood samples obtained from patients in five Chilean hospitals were identified and biotyped according to their phenotypic properties. They were also submitted to random amplified polymorphic DNA (RAPD) using eight randomly designed 10-mers and the core sequence of M13 phage (15-mers) as well as amplification of the spacer regions between 16S and 23S genes in the prokaryotic rRNA genetic loci. With some primers, RAPD discriminated between biotypes, whereas with others each isolate showed a particular profile. When amplification of spacer regions was performed, a clear correlation between patterns and biotypes was found. This last technique allowed correct biotyping of clinical isolates. Both genetic methods might be used for the identification of A. baumannii biotypes.

PubMed: 9141712

Affiliations:

Chili

Le document en format XML

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<term>Acinetobacter Infections (microbiology)</term>
<term>Bacterial Typing Techniques</term>
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<front><div type="abstract" xml:lang="en">Isolates of Acinetobacter baumannii (32 strains) from blood samples obtained from patients in five Chilean hospitals were identified and biotyped according to their phenotypic properties. They were also submitted to random amplified polymorphic DNA (RAPD) using eight randomly designed 10-mers and the core sequence of M13 phage (15-mers) as well as amplification of the spacer regions between 16S and 23S genes in the prokaryotic rRNA genetic loci. With some primers, RAPD discriminated between biotypes, whereas with others each isolate showed a particular profile. When amplification of spacer regions was performed, a clear correlation between patterns and biotypes was found. This last technique allowed correct biotyping of clinical isolates. Both genetic methods might be used for the identification of A. baumannii biotypes.</div>
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Serveur d'exploration MERS

Differentiation of Acinetobacter baumannii biotypes by amplification of 16S-23S rRNA intergenic spacer sequences.

Differentiation of Acinetobacter baumannii biotypes by amplification of 16S-23S rRNA intergenic spacer sequences.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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